2018 ACA CryoEM Workshop
The Cryo-EM Workshop: A Guide to High Resolution Structure Determination was held on July 21, 2018 as part of the American Crystallographic Association Annual Meeting in Toronto, Canada. Co-organized by Lori Passmore (MRC-LMB) and Wah Chiu (Stanford U), and sponsored by Douglas Instruments, Gatan, and ThermoFisher Scientific, the one-day workshop attracted over 130 attendees.
Cryo-EM has become increasingly popular among structural biologists and is now the method of choice for structure determination of proteins larger than ~200 kDa. It has been particularly successful for proteins that are difficult to crystallize, including membrane proteins, large assemblies and multi-protein complexes. The workshop provided a detailed overview of cryo-EM specimen preparation, image processing and building/refinement of atomic models, with focus on high-resolution single particle cryo-EM, and several hands-on tutorials. Materials from the workshop can be accessed through the links below.
Go to this public Box folder or this public DropBox folder to access talk pdfs and workshop zip files.
Slides/Handouts
| Instructor | Topic |
| Wah Chiu | Welcome and Introduction to Cryo-EM |
| Lori Passmore | Specimen Preparation for Cryo-EM |
| Chris Russo | Specimen Preparation, Data Collection and Radiation Damage || Russo&Henderson2018 |
| John Rubinstein | Image Processing — Intro, Background, Implementation |
| Marcus Brubaker | Image Processing — Bayesian Methods and Optimization |
| Ali Punjani | CryoSparc Image Processing Tutorial |
| Tom Terwilliger | Phenix tools for Cryo-EM |
| Matthew Baker | Model Building in Cryo-EM || Pathwalking Tutorial |
| Corey Hryc | Model Optimization Tutorial || zipfile |
| Corey Hryc | Model Validation Tutorial || zipfile |
Tutorial Software
These software packages are available on Mac, Linux and Windows.- UCSF Chimera is used to visualize/manipulate both map and model.
- Phenix (command line) is used to optimize the molecular model with respect to the experimental data.
- EMAN2 is used for Pathwalking as well as validation routines such as map/model FSC.
- COOT is used for model refinement in the Pathwalker Tutorial.
updated 24 July 2024